Dr. Martijn Verdoes from the Dept. of Tumor Immunology, in collaboration with the group of Prof. Matthew Bogyo at the Stanford School of Medicine developed a quenched activity-based probe (qABP) selective for the cysteine protease cathepsin S, a protease involved in antigen presentation.
qABPs are small molecule chemical tools which allow for the live cell visualization and subsequent biochemical characterization of the active pool of enzymes. In conjunction with this cathepsin S selective imaging tool a complementary pan-reactive cysteine cathepsin qABP was developed, which for the first time allowed for the live cell activity localization of cathepsin S relative to the other cathepsin family members in immune cells. As a proof of concept, vesicular compartments possessing exclusive cathepsin S activity were discovered in dendritic cells, but not in macrophages. This work was published in the Journal of the American Chemical Society.
Design of a highly selective quenched activity-based probe and its application in dual color imaging studies of cathepsin s activity localization. Oresic Bender K, Ofori L, van der Linden WA, Mock ED, Datta GK, Chowdhury S, Li H, Segal E, Sanchez Lopez M, Ellman JA, Figdor CG, Bogyo M, Verdoes M. J Am Chem Soc.;137:4771-7, 2015.
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